Study Title: ION-ZC1 Antimicrobial Study 2 MRSA Non-biofilm Comparing Vancomycin as a Control
Author: INDEVION Biotechnology Research and Development Ltd. Principal Investigator Dr. Zsolt Keresztessy, Ph.D. (2018).
Methods: Review of the antimicrobial activity of ION-ZC1 which was tested by the broth-based microdilution method following the standard CLSI guidelines (MRSA in non-biofilm conditions) against 10 isolates of methicillin-resistant Staphylococcus aureus. As a comparison (positive control), the MICs of vancomycin were determined using microdilution in broth. Vancomycin was tested at concentrations ranging from 0.06-32 mg/L.
Results: Final test concentrations ranged from 0.006 to 3.4% (v/v) for ION-ZC1. Minimum inhibitory concentrations (MIC) were determined after 24 hours as the lowest concentration at which a prominent decrease (50%) in turbidity was observed. The results showed that the MIC values for ION-ZC1 against the 10-methicillin-resistant Staphylococcus aureus isolates analyzed were in the range of 0.212 to 0.85% (v/v). For vancomycin, MIC values were in the range of 4 to 16 mg/L.
Conclusions: Comparing the average MIC value of ION-ZC1 of 0.531% (v / v) with the average MIC value of 10 mg / L for vancomycin A percentage solution calculation established twice the amount of vancomycin needed to create the same 50% turbidity in MRSA when tested compared to ION-ZC1. The results showed that the MIC values for ION-ZC1 against the 10-methicillin-resistant Staphylococcus aureus isolates analyzed were in the range of 0.212 to 0.85% (v/v).